Group clustering heatmaps reveal sample similarities, reflect intra-group variations, and guide the generation of biological hypotheses. They visually display gene or protein expression patterns through color changes.

Input

Relative species abundance table A tab-separated text file containing row and column headers, where each column represents a sample, and each row represents a taxonomic unit (gene names are unique and non-repeating).

Group Information Table

A tab separated text file containing row and column names, with column names fixed at (SampleID, Categorize). The first column is the sample name, and the second column is the corresponding group for the sample.

Output

At the bottom of the figure are the names of the classified samples. At the top of the figure is the group information for the samples. The phylogenetic tree above the figure indicates that, in specific samples, two or more taxonomic units have similar expression levels. The color intensity of the middle squares represents the relative expression levels of the corresponding taxonomic units in each sample—the redder the color, the higher the expression level.