LEfSe (LDA Effect Size) analysis is a statistical and bioinformatics method for high-throughput biological data that identifies differential features of microbial communities under various conditions

Input

Relative Abundance Table

A tab-separated text file that includes row and column headers, where each column represents a sample and each row represents a taxonomic unit (with taxonomic units being unique and non-repetitive); the second row indicates the group for each sample.

Output

lefse

The evolutionary branch diagram of differential species shows radiating circles from the inside out, representing the taxonomic levels from phylum to genus (or species). Each small circle on different classification levels represents a category at that level, and the diameter of the small circle is proportional to the relative abundance.